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24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

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UMR ECOSYS - Ecologie fonctionnelle et écotoxicologie des agroécosystèmes

Poster 3 : Periscope_Colza_racines

Poster 3

PERISCOPE: a new Phenotyping Experimental device for individual Root and shoot Investigations in reconStructed CanOPy until harvEst, under field-like conditions

Cultivars with improved nitrogen use efficiency (NUE) are central to face economic competitiveness and environmental sustainability of rapeseed. Under low n input, a higher NUE can be achieved by increasing N uptake efficiency (NUPE) and/or by improving N reserve use. NUPE results from root development and specific nitrogen uptake. Exploring the GxN variability of this efficiency remains challenging, as a reliable quantification of fine root fraction and N uptake remains difficult in the field. Our aim was to design and test a new semi-controlled culture system for growing rapeseed plants, able to fit the following requirements: i) quantitatively access to each plant fraction, including fine roots, ii) be relevant until harvest to estimate final yield components, iii) generate individual plants in a reconstructed canopy, with phenotype and yield similar to those of field grown plants, and iv) combine measurements at plant and crop scales. The PerIscoPe system consisted of individual columns of 1m high and 0,16m diameter, grouped by 24 into boxes of 1m3, placed outside and therefore submitted to field climate. Various combinations of substrates and n treatments were experienced in 2 locations. This new system was successful at collecting plant fractions, including fine roots, until harvest under field-like conditions. In addition, the canopies generated were similar to those obtained in the field. Rresults showed a good repeatability between plants and revealed a significant contribution of fine roots to total plant biomass (up to 23 %) This new experimental device proved to be effective to characterize the phenotypic responses of rapeseed to N limitation and should be helpful to screen large genotypic variability for NUE traits.

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